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Posted Job TitleResearch Specialist A/B (Department of Pulmonary, Allergy and Critical Care)Job Profile TitleResearch Specialist AJob Description SummaryThis Research specialist will manage several cellular and molecular biology projects within the laboratory including mammalian sterile tissue culture work (including human embryonic stem cells), nucleic acid work including DNA and RNA isolation. Q-RT-PCR, protein work and western blotting. They will also be responsible for several lab support roles including ordering and organization. They will coordinate with the rest of the team including the associated laboratory and institute colleagues to assist with human lung tissue work including distribution of tissue, and processing of human lung tissue for downstream experiments including histology, cellular sorting, cell culture, and molecular biology.Job Description
This Research specialist will manage several cellular and molecular biology projects within the laboratory including mammalian sterile tissue culture work (including human embryonic stem cells), nucleic acid work including DNA and RNA isolation, cloning, Q-RT-PCR, protein work and western blotting. They will also be responsible for lab support roles including ordering and organization. They will coordinate with the rest of the team including associated colleagues in other groups to assist with human lung tissue work including distribution of tissue, and processing of human lung tissue for downstream experiments including histology, cellular sorting, cell culture, and molecular biology.
Job Responsibilities
Sterile Tissue Culture work including human embryonic stem cell work, lentiviral production, organoid work, and cell line maintenance
Molecular biology including DNA, RNA and protein isolation and downstream applications including cloning and bacterial work
Cell isolation from primary human tissue including processing and antibody staining and flow cytometry and sorting
Ordering and lab organization
Training of additional staff as members are added to the laboratory in minor activities
Perform additional duties as assigned
In addition to the above:
Research Specialist A: Must have some laboratory experience working with molecular biology, cell culture or biochemical techniques. Must be willing to work with human tissue samples.
Research Specialist B: Prior experience with sterile cell culture and molecular biology with nucleic acids. Must have the ability to work independently and to adjust to rapidly changing needs of principal investigators and senior researchers. Must possess some but not all of the following laboratory skills: tissue culture, PCR, RNA isolation, RT-PCR, Western blots, flow cytometry, and immunostaining / immunofluorescence microscopy and analysis.
QUALIFICATIONS:
Research Specialist A: Bachelor’s Degree and 0 to 1 year of experience or equivalent combination of education and experience is required.
Research Specialist B: Bachelor’s Degree and 1 – 3 years of experience or equivalent combination of education and experience is required.
This position is contingent upon grant funding.
Job Location – City, StatePhiladelphia, PennsylvaniaDepartment / SchoolPerelman School of MedicinePay Range$36,368.00 – $57,600.00 Annual RateSalary offers are made based on the candidate’s qualifications, experience, skills, and education as they directly relate to the requirements of the position, as well as internal and market factors and grade profile. Affirmative Action Statement
Penn adheres to a policy that prohibits discrimination on the basis of race, color, sex, sexual orientation, gender identity, religion, creed, national or ethnic origin, citizenship status, age, disability, veteran status, or any other legally protected class.Special Requirements
Background check required after a conditional job offer is made. Consideration of the background check will be tailored to the requirements of the job.University Benefits
To learn more, please visit: https://www.hr.upenn.edu/PennHR/benefits-pay
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