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University of Tennessee Health Science Center Looking for Research Associate-Pharmacology Full Time
Description
Market Range: 11
Hiring Range: $4,474.01- $6,069.13/monthly
THIS IS A GRANT FUNDED POSITION.
JOB SUMMARY/ESSENTIAL JOB FUNCTIONS:
Under the direction of the Principal Investigator the Research Associate performs a variety of research activities on the role of ion channel-coding cDNAs and the resulting expressed proteins, and the modification of ion channel protein function by physiologically relevant lipids and pharmacological agents, in particular agents involved in substance use disorders such as alcohol and volatile inhalation agents.
DUTIES AND RESPONSIBILITIES:
- Conducts PCR, real-time PCR, construct vectors, DNA subcloning and in vitro transcription of recombinant DNA.
- Designs PCR primers, develop point mutants and chimeric channel subunits to determine amino acid participation in ligand recognition.
- Develops and maintains cell cultures using mammalian expression lines (CHO, COS, etc.,).
- Conducts cell transfection (both transient and stable), Western-blotting, immunohistochemistry, and other methods to identify protein expression.
- Conducts cDNA nuclear and cRNA cytoplasmic injections in frog oocytes.
- Conducts sucrose gradients for protein harvesting.
- Supervises and keep maintenance of all devices and materials related to recombinant DNA work and other techniques mentioned above.
- Maintains detailed log book of experiments.
- Supervises fellows, and junior employees in the laboratory.
- Performs other duties as required.
Qualifications
MINIMUM REQUIREMENTS:
EDUCATION: Ph. D. in Biochemistry, Molecular Biology or related field. (TRANSCRIPT REQUIRED)
EXPERIENCE: Three (3) years of experience in a biochemistry/molecular biology research laboratory. Documented proficiency (i.e., first author publications) with recombinant DN, in particular with mutagenesis and expression of long DNA sequences.
Job: Research Professional
Primary Location: US-Tennessee-Memphis
Organization: Pharmacology
Campus/Institute: Memphis
Schedule: Full-time
All qualified applicants will receive equal consideration for employment and admissions without regard to race, color, national origin, religion, sex, pregnancy, marital status, sexual orientation, gender identity, age, physical or mental disability, or covered veteran status.
Eligibility and other terms and conditions of employment benefits at The University of Tennessee are governed by laws and regulations of the State of Tennessee, and this non-discrimination statement is intended to be consistent with those laws and regulations.
In accordance with the requirements of Title VI of the Civil Rights Act of 1964, Title IX of the Education Amendments of 1972, Section 504 of the Rehabilitation Act of 1973, and the Americans with Disabilities Act of 1990, The University of Tennessee affirmatively states that it does not discriminate on the basis of race, sex, or disability in its education programs and activities, and this policy extends to employment by the University.
Inquiries and charges of violation of Title VI (race, color, national origin), Title IX (sex), Section 504 (disability), ADA (disability), Age Discrimination in Employment Act (age), sexual orientation, or veteran status should be directed to the Office of Equity and Diversity (OED), 910 Madison Avenue, Suite 826, Memphis, Tennessee 38163, telephone at 901-448-2133 or 901-448-7382 (V/TTY available). Requests for accommodation of a disability should be directed to the ADA Coordinator at the Office of Equity and Diversity.