Salk Institute for Biological Studies Hiring for Research Assistant II – NOMIS Center for Immunobiology and Microbial Pathogenesis, Dr Ye Zheng at La Jolla, CA Full Time

This position is responsible for duties in support of lab research projects in immunology.  Range of duties includes but is not limited to: managing a mouse colony; performing molecular biology techniques, immunology techniques; animal model research; data collection and analysis; assisting with lab maintenance such as sterilization of materials and preparation of solutions; maintaining adequate inventory of materials and equipment to ensure supplies for experimental needs. 

ESSENTIAL FUNCTIONS

(70%) Research Support

• Manage a mouse colony, including mouse handling, genotyping, and record keeping.
• Molecular biology techniques, including RNA and DNA extraction and analysis, cloning (restriction enzyme digestion, ligation, minipreps), PCR and RT-PCR, Western blot, gel electrophoresis, RNA-seq and ChIP-seq, CRISPR/Cas9 based genome editing.
• Immunology techniques, including isolation and culturing mouse primary T cells, retrovirus generation and transduction, flow cytometry, immunofluorescence, and immune disease models.

(30%) Laboratory Support

• Provides assistance to the lab manager to ensure the operation of essential equipment, making sure adequate supplies are available for routine experimental procedures, etc.
• Maintain a clean, orderly work area within the laboratory.
• Perform routine maintenance and make minor repairs to simple laboratory apparatus and instruments.
• Assist in the set-up and operation of simple laboratory apparatus and instruments; read and record instrument data; tabulate data; keep laboratory records.
• Perform an inventory of chemicals and supplies; order laboratory supplies and stock laboratory areas as required.
• Operate sterilization equipment ensuring appropriate conditions for the material being processed.
• Prepares various solutions and/or media according to established protocols.

EXPERIENCE

Required:

• Minimum 2 years’ experience or equivalent combination of education and experience.
• Demonstrated experience and application of molecular biology knowledge preferably in biochemistry and cell culture.
• Demonstrated experience and application of immunology knowledge preferably in T cell culture and related assays.

Preferred:

• Experience working with mice.
• Experience as a lab technician or research assistant, or equivalent combination of education and experience.
• Experience with general lab maintenance, including cleaning, stocking supply inventory, and ensuring the maintenance of a sterile environment.

EDUCATION

Required:

Bachelor’s degree in Biological Sciences or related scientific discipline required. Bachelor’s degrees in a different discipline and a minimum of 4 years of experience in a scientific role will be considered in lieu.

Preferred:

• Master’s degree in biological sciences or related field.

SKILLS AND ABILITIES

Required:

• Theoretical and hands on knowledge of tissue culture, molecular biology techniques, biochemistry, physiology and cell biology.
• Ability to carry out new experimental procedures with accuracy and efficiency. Demonstrated ability to follow and interpret verbal and/or written instructions, including follow-up on previously begun procedures. Demonstrated ability to record data accurately and enter into appropriate medium.
• Demonstrated ability to calculate and prepare chemical solutions accurately.
• Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions, recommendations, and plans of action.
• Skill in the operation and maintenance of various lab equipment.
• Highly organized, effective at multitasking, adept at juggling changing priorities.
• Demonstrated knowledge and experience with both PC and MAC operating systems, standard business office equipment and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.
• Proficiency with common office software applications as well as software applications used for research, recording, and analysis.
• Proven ability to work both independently and also as part of a collaborative team.
• Excellent communication skills, both oral and written.
• Strong interpersonal skills, including tact, diplomacy, and flexibility. Demonstrated ability to interact effectively with diverse groups. Demonstrated skill in establishing and maintaining effective working relationships with personnel at all levels.

 Salary range for this position is $21.15 to $25 per hour

SPECIAL CONDITIONS OF EMPLOYMENT

• Must be willing to work in an animal-related research environment.
• Satisfactory completion of the Institute’s background investigation.
• Willing to sign a confidentiality agreement.
• Able to work a flexible schedule based on research needs and deadlines.

PHYSICAL REQUIREMENTS/MENTAL ACTIVITIES/ENVIRONMENTAL CONDITIONS

The incumbent in this position will be constantly adjusting focus, seeing, and working indoors.